Unlike protein, that did not accumulate during the culture trial,lipids, more particularly n-3 HUFA as DHA and EPA gradually built up in the rotifer tissue during the first culture cycle and stabilized after 4 days. After this first cycle the n-3 HUFA, DHA and EPA in the rotifer
tissue reached identical concentration as in a classic enrichment procedure of 2–3 h. In this combined culture-enrichment technique the essential fatty acids are not only boosted in the digestive tract of the rotifers but a substantial amount will be stored in the tissue and reproductive organs where it is passed on to the neonates