Supplementation of biotin increased milk production and
protein percent and yield, but did not exert effect on DMI,
milk fat and plasma triglyceride and NEFA. The supplemental
biotin increased the plasma level of biotin, with no difference
between the 20 and 40 mg/d group. Moisture content and
hardness of hoof were not affected by biotin supplementation,
but the hoof of dairy cows with supplemental biotin