PCR analysis with cry2Aa specific primer resulted in the amplifica- tion of 498 bp. However when full ORF was amplified, sequenced and digested in silico and in vitro, it showed difference from other reported cry2A crystal protein genes. These results suggested that the isolate carried some cry2A related gene but not the cry2Aa gene. The sequence was named as cry2Al1, a new cry2A haplotype, by B. thuringiensis delta-endotoxin nomenclature committee.