DNA metabarcoding was carried out on all semples using the D1/D2 variable region of the ribosomal LSU with the eukaryotic primers NLF184(TACCCGCTGAAYTTAAGCATAT;modified from Van der Auwera et ak. 1994)and (AGACTCCTTGGTCCRTGT;modified from NLR818,Van der Auwera et al. 1994).
After in silico tests using TestPrime