Mass spectrometry with instrumental chromatography
Chromatography instruments (chapters 11 to 13) are instruments that separate and quantitate samples of complex mixtures with surprising ease accuracy in a very short time. The separation occurs in a column, which is a tube that contains a stationary material phase, either a liquid or solid, that selectively impedes the progress of the mixture components as they move thorugh it. This forward movement occurs because a second phase, a moving has or liquid phase called the mobile phase , is passing through the column and also influences the rate of movement of the mixture components. Mixture components then emerge from the end of the column one at time and are electronically detected and measured one at a time, amass spectrometer can be used as the detector system.
When the mobile phase is a gas, the technique is called gas chromatography-mass spectrometry (GC-MS). In this case , the mixture is in the gas phase as it moves through the column, and the individual separated mixture components emerge in the gas phase and mix with the gaseous mobile phase , which is usually helium. When the mobile phase is a liquid, or a mixture of liquids, the technique is called liquid chromatography-mass spectrometry (LC-MS). In this case, the mixture is in the liquid phase as it moves through the colomn and individual searted mixture components emerge dissolved in this liquid phase.
In either case, there is a need to eliminate significant amounts of gas or liquid as the sample passes into the high-vacuum environment of the mass spectrometer. Thus a special interface is needed to accomplish this but, indeed, reliable interfaces have been invented as evidenced by the extensive use of these techniques in modern analytical laboratories. See figure 10.21. also, see additional discussions in chapters 12 and 13 (sections 12.6.7 and 13.6.6), as well as workplace scene 13.5.
Mass spectrometry offers a special advantage over other detection schemes for instrumental chroma-tography because the mass spectra of the mixture components are acquired, providing a very powerful qualitative analysis tool for these components.
Mass spectrometry with instrumental chromatographyChromatography instruments (chapters 11 to 13) are instruments that separate and quantitate samples of complex mixtures with surprising ease accuracy in a very short time. The separation occurs in a column, which is a tube that contains a stationary material phase, either a liquid or solid, that selectively impedes the progress of the mixture components as they move thorugh it. This forward movement occurs because a second phase, a moving has or liquid phase called the mobile phase , is passing through the column and also influences the rate of movement of the mixture components. Mixture components then emerge from the end of the column one at time and are electronically detected and measured one at a time, amass spectrometer can be used as the detector system. When the mobile phase is a gas, the technique is called gas chromatography-mass spectrometry (GC-MS). In this case , the mixture is in the gas phase as it moves through the column, and the individual separated mixture components emerge in the gas phase and mix with the gaseous mobile phase , which is usually helium. When the mobile phase is a liquid, or a mixture of liquids, the technique is called liquid chromatography-mass spectrometry (LC-MS). In this case, the mixture is in the liquid phase as it moves through the colomn and individual searted mixture components emerge dissolved in this liquid phase.In either case, there is a need to eliminate significant amounts of gas or liquid as the sample passes into the high-vacuum environment of the mass spectrometer. Thus a special interface is needed to accomplish this but, indeed, reliable interfaces have been invented as evidenced by the extensive use of these techniques in modern analytical laboratories. See figure 10.21. also, see additional discussions in chapters 12 and 13 (sections 12.6.7 and 13.6.6), as well as workplace scene 13.5.Mass spectrometry offers a special advantage over other detection schemes for instrumental chroma-tography because the mass spectra of the mixture components are acquired, providing a very powerful qualitative analysis tool for these components.
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Mass spectrometry with instrumental chromatography
Chromatography instruments (chapters 11 to 13) are instruments that separate and quantitate samples of complex mixtures with surprising ease accuracy in a very short time. The separation occurs in a column, which is a tube that contains a stationary material phase, either a liquid or solid, that selectively impedes the progress of the mixture components as they move thorugh it. This forward movement occurs because a second phase, a moving has or liquid phase called the mobile phase , is passing through the column and also influences the rate of movement of the mixture components. Mixture components then emerge from the end of the column one at time and are electronically detected and measured one at a time, amass spectrometer can be used as the detector system.
When the mobile phase is a gas, the technique is called gas chromatography-mass spectrometry (GC-MS). In this case , the mixture is in the gas phase as it moves through the column, and the individual separated mixture components emerge in the gas phase and mix with the gaseous mobile phase , which is usually helium. When the mobile phase is a liquid, or a mixture of liquids, the technique is called liquid chromatography-mass spectrometry (LC-MS). In this case, the mixture is in the liquid phase as it moves through the colomn and individual searted mixture components emerge dissolved in this liquid phase.
In either case, there is a need to eliminate significant amounts of gas or liquid as the sample passes into the high-vacuum environment of the mass spectrometer. Thus a special interface is needed to accomplish this but, indeed, reliable interfaces have been invented as evidenced by the extensive use of these techniques in modern analytical laboratories. See figure 10.21. also, see additional discussions in chapters 12 and 13 (sections 12.6.7 and 13.6.6), as well as workplace scene 13.5.
Mass spectrometry offers a special advantage over other detection schemes for instrumental chroma-tography because the mass spectra of the mixture components are acquired, providing a very powerful qualitative analysis tool for these components.
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