Bioactivities of latex tapping from newly and repeated wounded papaya fruit were investigated. Four types of papaya latex including the first time tapped liquid latex (FL), the first time clotting latex (FC), repeated tapped liquid latex (RL) and repeated tapped clotting latex (RC) were used. All papaya latex samples were fractionated with ultracentrifugation employing 3, 10 and 30 kDa MW cut-off membranes. The highest protease activity of 10,789 units was found in the crude latex of RL, while the highest protein content of 300.6 mg was observed in that of FC. After fractionation, the protease activities (more than 80% of crude latex) and proteins were found in the fractions of MW >30 kDa and almost absent in the fractions of MW lower 10 kDa. On the other hand, the potential antioxidant activities with IC50 1.13-1.64 µg/mL (assayed by ABTS method) were obtained in the fractions of low MW, especially those of ≤ 3 kDa. The crude latex and >30 kDa fractions showed high absorbance readings at UVB range and could be comparable to that of oxybenzone at around 282 nm. This study showed potential bioactivities of papaya latex extract for food and cosmetic applications.