L-Asparagine, L-proline, CuSO4·5H2O and KH2PO4 were used to
reduce phenolics in callus induction medium and improve callus induction
rate. Eight different media (Table 1)were tested to find out the optimal
concentration of CuSO4·5H2O and the best combination of
additional compounds for callus induction. Twenty-five immature
embryos (IEs) of SA281 were isolated and placed on callus induction
medium. Three Petri dishes were used as three replicates for each
medium treatment. Callus induction rates were calculated, and callus
fresh weight per IE was recorded two weeks after initiation.
2.3. Regeneration