2.3.3. Daily sperm production per testis, sperm number and transit time in
the epididymis
Right testis was decapsulated and the caput/corpus and cauda
segments from epididymis were separated. Homogenization-resistant
testicular spermatids (stage 19 of spermiogenesis) and sperm in the
caput/corpus epididymis and cauda epididymis were assessed as described previously by Robb et al. [26], with adaptations of Fernandes
et al. [27]. Mature spermatids were counted in a Neubauer chamber. To
calculate daily sperm production (DSP), the number of spermatids at
stage 19 was divided by 6.1, which is the number of days in one seminiferous
cycle when these spermatids are present in the seminiferous
epithelium. Sperm transit time through the epididymis was determined
by dividing the number of sperm in each segment by the DSP