ater to remove surface debris, dissected into small
segments; approximately 1 × 1 cm, and surface-sterilized by sequential
immersion in 5% hypochlorite for 1-2 minutes followed by 75% ethanol for 30
seconds. Surface sterilised segments (4 segments/plate) were selected, plated
out on water agar amended with 0.5 gmstreptomycin, 2000 units penicillin G
per plate and 0.3 g/L Rose Bengal. Ten plates were used for each plant part