Separation of curcuminoids by TLC: Methanol extracts were tested on TLC for presence different curcuminoids. The TLC pre-coated silica gel. Plate were developed using glass beaker , which was pre-saturated with mobile phase for 20 min and each plate was developed up to a height of about 6.8 cm. chloroform : methanol mobile phase was used with composition 95: 5. After development, plates were removed and dried. Spots were analyzed.