2.2.2. Three phase partitioning
Slurry was prepared by dispersing 1 g kokum kernel powder in
16 ml distilled water by gentle stirring on a magnetic stirrer. The
pH of slurry was noted and then adjusted to the desired value by
adding 0.1 N HCl or 0.1 N NaOH. Weighed amount of ammonium
sulphate (10 to 60% w/v of slurry) was added to the slurry prepared
and vortexed gently, followed by addition of measured amount of
t-butanol (t-butanol to slurry ratio = 0.5:1 to 3:1). This slurry of salt