Some background interference was observed in the low MW region of the blot, and a single area of immunoreactivity was observed in the basic high molecular weight region.
This protein was not able to be identified and is indicated by arrows in Fig. 2e.
Further to this, the proteins 50S ribosomal protein L1/L12 (FTT_0143), hypothetical membrane protein (FTT_1778c), and acetyl CoA carboxylase (FTT_0472) were immunoreactive with 8 or more patient sera screened and with none of the control sera. Of note, the proteins FTT_1778c and FTT_0143 focus to discrete spots on 2D-PAGE within close proximity of one another.