1) Transplant stock culture of G. lucidum based on Thailand Mushroom Culture Collection on PDA Petri dish
incubated in room temperature for 5 days
2) Apply an apparatus for punching cork for 0.5 cm diameter of outer area of colony
3) Soak sorghum seeds for 12 hours and sieve for draining and dry in the air
4)Boil in water for 30 minutes then percolate with filter and expose in the room temperature till cool 5) Contain in 250 ml flask with only 2/3 part of the bottle then sterilize in autoclave at 121oC with 15 psi. for 30 minutes and
6) Stir substrate in item 2)upon the bottle of sorghum cool down, to incubate in the room temperature for 5 days resulting in the sorghum spawn