For the agar diffusion assay, overnight cultures were diluted using PBS to 1 × 107 CFUs/ml. Aliquots of 100 μl
of yeast suspension were spread onto Mueller Hinton agar (MHA) medium supplemented with 2% glucose. To
examine the antifungal activity of each combination of partner drugs and FLC, cellulose disks impregnated with
16, 32, and 64 μ g of the solubilized drugs and 4 μ g of FLC and the control disk impregnated with the corresponding
solvent were placed onto YPD agar plates. After 48 h of incubation at 30 °C, the horizontal and vertical diameters
of the growth inhibition areas were recorded.