2.4 Inhibition of spore germination on mango fruit Spore suspension of C. gloeosporioides was prepared from 10-day-old PDA culture at concentration of 5.4×108 spores/mL. Cell suspension of the yeast I. orientalis was prepared from 4-day-old PDA culture at concentration of 4.3×108 cells/mL. For surface sterilization mangoes, cv. Nam Dok Mai, were immersed in 1% sodium hypochlorite, for 5 min. and then rinsed with sterile distilled water and left to dry. Suspension of the fungal pathogen and yeast was inoculated on the mango fruit in the same marked area, 1 mL each. For the control treatment, only a suspension of the fungal pathogen was inoculated. The mangoes were incubated in a plastic box for 5 days at room temperature. Pieces of mango peel were then processed for scanning electron microscope observation. Spore germination in the presence of the yeast was investigated.