Spots with Rf values identical to standard IAA were identified under UV light (254 nm), and sprayed with
Salkowski’s reagent (Ehmann, 1977) and Ehmann’s reagent (mixed 1 volume of 50 mL of 0.2% (w/v) p-dimethylaminobenzaldehyde in concentrated HCl combined with 50 mL of absolute ethanol and 3
volumes of 2.03 g of FeCl3.6H2O in 500 mL of water and 300 mL of conc. H2SO4).