and stirring rate were controlled through an electronic control panel.Temperature was monitored by an infra-red temperature sensor and controlled by feedback to the regulator. The extraction
flask was placed in the microwave resonance cavity and connected
to the cooling system through a hole at the top of microwave accelerated reaction system.
A known amount of enzymes and 10 g of ground seed materials
(80-mesh) were introduced into the reaction flask (100 mL) with
60 mL water and pH was adjusted to 5 with citric acid. The reaction
flask was placed symmetrically in the microwave resonance cavity
and connected to the cooling system. A magnetic stirrer bar was
placed in the flask to ensure thorough mixing (200 rpm). Based
on the results of our preliminary experiments (data not shown),
the particle size 80-mesh, liquid/solid ratio 6:1 (mL/g), pH 5 and
stirring speed 200 rpm of enzymes.