The reaction
mixture containing 0.4ml of 0.2% azocasein (dissolved in
20mM NaPO4 buffer, pH 5.7) and 0.5ml of the extracted extracellular
enzyme was heated at 55 ◦C for 60min. The mixture
was cooled in ice for 5min, then 0.5ml 10% trichloroacetic
acid was added to.