In this study, we’re activated hormones by inject 17β-estradiol 2 mg/ 1 kg of immaturity white perch, the average weigh is 13 g, Activated every 6 hours for 2 weeks. After that used syringe no. 25G x ½” for collect blood samples from tail and suspend in 1.5% EDTA before centrifuge in speed 5,000 rpm for 5 minutes.