Banana production has been severely hindered by fusarium wilt disease. The application of organic
amendments to soil has been proposed as a strategy for management of the soil borne disease. The oneyear effects of different composts and bio-organic fertilizer (BIO) on banana fusarium wilt disease
incidence, crop yield, crop quality, and the soil microflora were investigated infield condition. A plate
count method, real-time PCR, and PCR-DGGE were used to investigate the soil microflora. The BIO
application more effectively controlled fusarium wilt disease. The highest total soluble sugars (TSS) to
titratable acidity (TSS/TA) ratios, banana yield, culturable and total soil bacteria populations, and culturable actinobacteria population were observed from the BIO treatment. The ratio of bacteria to fungi (B/
F) and actinomycetes to fungi (A/F) values from the plate count method and the B/F value from the realtime PCR analysis were also highest in the soils collected from the BIO application. Based on PCR-DGGE
results, the soil bacteria structure was significantly altered in the soils collected from BIO treatment
compared to the other treatments. All the results confirmed that application of bio-organic fertilizer
could more effectively control fusarium wilt disease in field conditions by improving soil microbial
communities