Re-sampling the same dogs during the subsequent rainy season allowed calculating the incidence rates of Babesia
infections in the three regions. The Real Time PCR detected only one new case of infection among 68 negative dogs
in Lavras and only one new case among 25 negative dogs in Belo Horizonte, resulting incidence rates of 1.5% and
4.0%, respectively. However, a much higher incidence rate (24.1%) was observed in Nanuque, where 14 new cases
were identified among 58 negative animals.