Leaf samples were extracted in two volumes
of 0.2 MTris HCl buffer (pH 7.8) containing 14 mMb-mercaptoethanol.
b-1,3-glucanase activity was assayed by incubating
62.5 ll of the leaf homogenate containing the enzyme for 2 h
at 40 C with 62.5 ll of 4% laminarin.