After extensive washing with the working buffer, the steady-state response of the biosensor was determined.
The level of inhibition due to the action of a definite pesticide was evaluated by comparison of the steady-state
levels of the biosensor before and after treatment with a pesticide solution. To reactivate the inhibited enzyme, the
biosensor was immersed for 10 to 60 min in a vigorously stirred reactivation solution consisting of a saturated solution
of pyridine-2-aldoxime methiodide PAM-2 in PBS