Then, once the primers for the tuf gene were designed, these were used for the amplification of all Bacillus strains studied, this resulting in the amplification of a single product of 791 bp. Afterwards, sequencing was performed in both directions, and the primers used for PCR were also employed for the sequencing of both strands of the PCR products. Subsequently, the sequences were analysed for each species and deposited in the GenBank sequence database