however, thetransparencyofthesamplesincreasedatTc2ascompared to Tc1. It was considered that phase transition progressed also between Tc1 and Tc2, as similar to To and Tp. Such appearance changes were observed in both SA and IMA samples.Therefore, it appear edthat the major peak between To and Tc2 in the DSC curve of adipose tissues represented the heat transition of fat in the tissues.
The glass-capillary tube method is generally used follow ingfatextractionforanalysisofthemelting poin tofporkfat (Dugan, 1987). The capillary tube method, however, requires many steps for analysis such as fat extraction, capillary preparation, and determination. Additionally, the melting point in the capillary tube method is a subjective visual inspection. Therefore, use of capillary tube method has been difficult to maintain objectivity and repeatability of determination of the melting point. Waugh and Small (1984) successfully analyzed rabbit lipid-rich tissue, including adipose tissues, by DSC for in situ tissue lipid characterization, and, thus it was hypothesized that the direct application of the DSC to porcine adipose tissue would be a useful and simple method for analysis of pork fat melting pattern because it requires only one step of sample preparation and allows for objective detection of phase transition. To investigate this application of DSC, we obtained DSC curves by direct analysis of porcine adipose tissues. The shape of DSC curves of adipose tissues was similar to those in the extracted fat, but there were some differences in detail. In tissue level DSC curve, a major peak between To and Tc2 was observed, and the changes in appearance of adipose tissue was associated with the heat transition, that is to say fat melting was