The PCR amplification conditions were as
follows: 94°C for 5 min, followed by 35 cycles of 94°C for 35 sec,
52°C for 55 sec, and 72°C for 1 min, with a final extension step at
72°C for 10 min (for ITS). For RPB2 gene fragments, 94°C for 5 min
(1 cycle); 94°C for 30 s, 55°C for 30 s, and 72°C for 90 s (35 cycles),
and then 72°C for 10 min (1 cycle).