The antioxidant activity was measured using a modified 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay [29–31]. For the ABTS assay, equal volumes of 3 mM ABTS radical and 8 mM potassium persulfate were allowed to react in the dark for at least 16 hours at room temperature to prepare the stock solution. Five mL of the stock solution was mixed with 145 mL of phosphate buffer (pH 7.4) to make the working solution. In a 96-well microplate, 290 μL of the ABTS working solution was mixed with 10 μL of extract and allowed to react for 30 minutes. The absorbance was measured at 734 nm using a microplate reader. The antioxidant activity of the samples was expressed as milligrams of Trolox equivalents per gram of sample (mg TE/g).