Collected fungal cells were multiplied in Biotechnology Laboratory, Department of Chemistry and Biochemistry,University of Agriculture, Faisalabad, Pakistan. For immobilization studies, live fungal cells (dry weight = 1g) were suspended in 2% sodium alginate solution to form a homogeneous mixture. Beads were formed by introducing this solution drop wise into the CaCl2 (0.1M) solution. The biosorbent beads were preserved in 50mM CaCl2 solution till further use.