To evaluate the applicability of the proposed method, four composed honey samples were obtained in the Santa Maria (Brazil) region from a local producer. Each sample consisted of portions collected from 12 different hives. The sampling was made in the years of 2011 and 2012, from spring to summer, the higher production period for honey in south of Brazil. The hives were located close to eucalyptus, peach, orange, grape and soybean cultivation areas. The samples were stored in 1 L glass containers,maintained at room temperature and analyzed before 30 days.