Pumpkin crude extract (PCE) was extracted from pumpkin powder with water and 95%
ethanol at 60C. Several components were isolated and further purified by solvents puri-
fication and dialysis as well as column chromatography. The anti-diabetic activities of the
hypoglycemic components extracted from pumpkin were identified using diabetic model
mice which were induced by alloxan intraperitoneal injection. The diabetic mice were
treated with pumpkin extracts by intraperitoneal injection at dosages of 100, 200 and
400 mg/kg body weight. Blood samples for glucose assays were taken from the diabetic
mice before injection and 4, 7 and 11 hours after injection. The results showed that the
blood glucose levels in the diabetic mice were significantly reduced by PCE-C from
15.32 4.38 mM to 5.77 1.46 mM ( p < 0.001) in 7 hours. PCE-C was further purified by
deproteinization, dialysis and Sephadex G-100 column chromatography and a fraction as
PCE-F was collected. Seven hours after PCE-F injection at a dosage of 200 mg/kg body
weight, blood glucose levels dropped significantly from 15.90 3.21 mM to 7.19 2.54 mM
( p < 0.01). PCE-F was identified by gas chromatography as a polysaccharide consisting of
heterogeneous monosaccharides, such as glucose, galactose, arabinose and rhamnose.
Copyright ª 2013, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan
LL
Pumpkin crude extract (PCE) was extracted from pumpkin powder with water and 95%ethanol at 60C. Several components were isolated and further purified by solvents puri-fication and dialysis as well as column chromatography. The anti-diabetic activities of thehypoglycemic components extracted from pumpkin were identified using diabetic modelmice which were induced by alloxan intraperitoneal injection. The diabetic mice weretreated with pumpkin extracts by intraperitoneal injection at dosages of 100, 200 and400 mg/kg body weight. Blood samples for glucose assays were taken from the diabeticmice before injection and 4, 7 and 11 hours after injection. The results showed that theblood glucose levels in the diabetic mice were significantly reduced by PCE-C from15.32 4.38 mM to 5.77 1.46 mM ( p < 0.001) in 7 hours. PCE-C was further purified bydeproteinization, dialysis and Sephadex G-100 column chromatography and a fraction asPCE-F was collected. Seven hours after PCE-F injection at a dosage of 200 mg/kg bodyweight, blood glucose levels dropped significantly from 15.90 3.21 mM to 7.19 2.54 mM( p < 0.01). PCE-F was identified by gas chromatography as a polysaccharide consisting ofheterogeneous monosaccharides, such as glucose, galactose, arabinose and rhamnose.Copyright ª 2013, Food and Drug Administration, Taiwan. Published by Elsevier TaiwanLL
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