Nine hundred and thirty-five isolates were screened and 657
further tested for hydrolytic enzymes and siderophores production.
In order to completely characterize, 30 bacteria isolates subjected
to DNA analysis, a computer-assisted evaluation of BOX-PCR
generated fingerprint was carried out using GelComparII version
4.5 (Applied Math’s BVBA, Kortrijk, Belgium). The analysis was performed
using Pearson correlation applied to densitometric curves
formed by the fingerprints (Rademaker and De Bruijn, 1997). We
performed Clustering analysis using the unweighted pair grouping
method based on arithmetic averages (UPGMA) in order to determine
the population structure of the isolates. After deleting the
isolates with same BOX-PCR fingerprint and the same assessment
of potential biological control characteristics, the selected bacterial
strains were identified by sequencing their 16s rRNA gene, and the
sequences were compared, using the basic local alignment search
tool (BLAST), with the reference sequences in the Nucleotide
Sequence Database of NCBI (National Center for Biotechnology
Information).