The purpose of the present study was to compare the 2 official
methods for proline determination in honey, the IHC method and
the AOAC method, with the original Ough method (1969) and to
choose the bestmethod in terms of accuracy and time saving. In order
to verify the performance of the best method chosen and optimized
for the waiting time for absorbance recording, quality parameters
such as accuracy, precision, limit of detection (LOD), limit of quantification
(LOQ), and linearity range were also evaluated.
The method was applied to 43 unifloral honey samples from the
Marche region, Central Italy.