White shrimps (Litopenaeus vannamei) with a size of
90 shrimps/kg were obtained from shrimp farms in Songkhla
Province, 24-36 hours after they were collected. Within an
hour the shrimps were placed in ice with an ice/shrimp ratio
of 2:1 (W/W) and transported to the Department of Food
Technology, Prince of Songkla University (PSU), Hat Yai,
Songkhla. Upon arrival, the shrimps were washed with clean
water, deheaded, peeled and ground with a grinder (Moulinex
Masterchef-350, La Defense, France) for 10 seconds. The
ground shrimps were packed in polyethylene bags and kept
in ice until used. The ground shrimps were stored not longer
than 12 hours.