In the current study, betacyanin content was significantly higher at pH 5 compared to pH 7 (p≤0.05) (Table 2), in accordance to that ofWoo et al. (2011). No significant difference was observed at pH 3, 4 and 6 (p>0.05) (Table 2). Since there are limited types of yeasts, molds and bacteria that are able to grow in low pH juices of pH 3.3–4.0 (Bull et al. 2004), pH 4 was selected for subsequent studies for preservation purposes. Under acidic conditions, decarboxylation of betanin was observed especially when heated at approximately pH 3–4 (Huang and von Elbe 1987). Dehydrogenation to the yellow neobetanin was also