To confirm the
genotypic distinction of strains WS4599, WS4601T and
WS4602 from recognised species of the genus Micrococcus,
sequences of the protein-encoding recA gene were used as a
basis for further phylogenetic analyses. recA gene fragments
(844 bp) were amplified by using the degenerated primers
GPRA-UF2 and GPRA-UR2, which were originally designed
by van Waasbergen et al. (2000) to amplify recA gene
fragments of species of the genus Arthrobacter. PCR
products were obtained for the three novel strains and the
type strains of Micrococcus lylae, M. flavus and M.
yunnanensis