2.3. Evaluation of the Genomic DNA Isolated in Downstream Applications
The genomic DNA obtained with the presented methodology was of high quality regarding all
standards employed. However, and as described by different authors, the quality and total DNA
contents provided by NanoDrop do not accurately represents the quantity of DNA that is efficiently
amplifiable by PCR [18,19]. In order to analyze the quality of amplifiable DNA, we PCR-amplified
amplicons for the histone H3 gene in all samples. We also performed a random PCR that would ideally
generate several DNA segments, like RAPDs, since this technique covers the entire genome.