Ribosomal internal transcribed spacer (ITS) amplified by PCR
from nail clippings hybridized only with Candida albicansspecific oligonucleotide probe immobilized on a DNA chip.
(A) Layout of oligonucleotide probes on the array (1.1 by 0.9
cm). The probe “ PC” was positive control and designed on the
basis of the ITS 2 region of Saccharomycodes ludwigii BCRC
21378. Probes coded“ NC” were negative controls (tracking
dye only). Probes coded“ M” were ITS 4 labeled with digoxigenin at its 5L end and were used as position markers. CAB5
is the oligonucleotide probe designed for Candida albicans.
(B) The array showed the positive results at CAB5 and PC probes