Chemical analyses for aflatoxins were performed on autopsy specimens from 23 Thai children who died with acute encephalopathy and fatty degeneration of the viscera (EFDV) and from 15 children and adolescents who died from unrelated causes. Aflatoxin B1 was found in one or more specimens from 22 of the 23 EFDV cases. The highest levels detected were 93 μg B1/kg in a liver specimen, 123 μg/kg in stool, 127 μg/kg in stomach and intestinal contents and 8 μg/ml in bile. Trace amounts were detected also in brain, kidney and urine. Aflatoxin B2 was indicated, but not confirmed, in 12 specimens of brain, liver, kidney, stool and stomach and intestinal contents. A blue fluorescent spot with the chromatographic properties of aflatoxin M1 was found in trace amounts in two urine specimens and a spot similar to that of aflatoxin B2 was found in another. None of the urine specimens from healthy control children contained any of the aflatoxins, but very small amounts of aflatoxin B1 (1–4 μg/kg tissue) could be demonstrated in some autopsy specimens from 11 of the 15 control subjects.
Chemical analyses for aflatoxins were performed on autopsy specimens from 23 Thai children who died with acute encephalopathy and fatty degeneration of the viscera (EFDV) and from 15 children and adolescents who died from unrelated causes. Aflatoxin B1 was found in one or more specimens from 22 of the 23 EFDV cases. The highest levels detected were 93 μg B1/kg in a liver specimen, 123 μg/kg in stool, 127 μg/kg in stomach and intestinal contents and 8 μg/ml in bile. Trace amounts were detected also in brain, kidney and urine. Aflatoxin B2 was indicated, but not confirmed, in 12 specimens of brain, liver, kidney, stool and stomach and intestinal contents. A blue fluorescent spot with the chromatographic properties of aflatoxin M1 was found in trace amounts in two urine specimens and a spot similar to that of aflatoxin B2 was found in another. None of the urine specimens from healthy control children contained any of the aflatoxins, but very small amounts of aflatoxin B1 (1–4 μg/kg tissue) could be demonstrated in some autopsy specimens from 11 of the 15 control subjects.
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