After the overnight incubation
at 37 °C, 0.1 ml aliquots were inoculated into tubes containing
10 ml Rappaport Vassiliadis (RV) broth and incubated for 48 h at
42 °C. Xylose Lysine Deoxycholate (XLD) agar plates were inoculated
from each of the RV broths and incubated for 18–24 h at 37 °C. Up
to five suspect colonies with typical Salmonella morphology were
confirmed biochemically by inoculating into Lysine Iron Agar (LIA)
and Triple Sugar Iron Agar (TSIA) slopes with confirmation carried
out usi ng specific Salmonella O and H agglutinating antisera (Difco
2537–47).