The replacement for traditional cultivation techniques of strawberry fruit with soilless systems has steadily grown over the past decades in the region of Huelva (Spain) and elsewhere. Soilless cultivation of strawberry fruit is thought to have numerous environmental advantages over traditional systems, since water and nutrients are used in a more sustainable manner and the vulnerable soil reserves are not degraded. In this context, it is well recognised that certain pre-harvest practices or conditions may affect the taste and health-related composition of strawberry fruit as well as alter plant productivity. Nonetheless,the present study has shown that despite some minor differences in fruit yield, a number of different substrates with different physicochemical properties may be employed during soilless cultivation of strawberry fruit without affecting the final fruit quality or composition. As reported in other studies cultivar differences accounted for most of the variability observed in this study.