Preparation of material
In this new strategy, N-acetylmuramide glycanohydrolase is introduced as the critical enzyme
which will react with the polymeric matrix of the biofilm, reduce its adherence and make the biofilm
detach from the surface. In this study, the optimal pH and temperature for reaction is 101 and
4528C, respectively. The temperature of 4528C is used throughout the whole application procedure.
The material was processed in the following manner.