This work aims at evaluating encapsulation and controlled release of vitamin B2 from alginate/chitosan
nanoparticles. Ionotropic polyelectrolyte pre-gelation was used as production method being chitosan and
alginate used as main materials. Nanoparticles were characterized in terms of average size, polydisper-
sity index (PDI), zeta potential and vitamin entrapment efficiency. The average size for alginate/chitosan
nanoparticles was 119.5 ± 49.9 nm for samples without vitamin B2 and 104.0 ± 67.2 nm with the encapsu-
lation of vitamin B2, presenting a PDI of 0.454 ± 0.066 and 0.319 ± 0.068, respectively. The nanoparticles
showed encapsulation efficiency and loading capacity values of 55.9 ± 5.6% and 2.2 ± 0.6%, respectively.
Release profiles were evaluated at different conditions showing that the polymeric relaxation was the
most influent phenomenon in vitamin B2 release. In order to study their stability nanoparticles were
stored at 4 ◦C being particles sizes and PDI evaluated during 5 months showing the results that vitamin
B2-loaded nanoparticles are more stable (in terms of size and PDI) than nanoparticles without vitamin
B2.