Given the positive findings regarding the inhibitory activity of B. rotunda against biofilm formation of several oral pathogens, Rukayadi et al. further investigated its inhibitory properties against enterococcal biofilm formation. Enterococci, particularly E. faecalis and E. faecium, are common Gram-positive cocci found typically in the intestinal tract and are known to cause intestinal and urinary infections. By employing the CSLI guidelines, the MIC of panduratin A was found to be 2 μg/mL while the MBC that effectively killed all the enterococci isolates was 8 μg/mL. At 4X MIC, the compound caused bactericidal effect after 30 mins of incubation, as determined by time-kill curve study. The MIC of panduratin A was also lower than that of other commercial antibacterial agents such as ampicillin (256 μg/mL), tetracycline (64 μg/mL), gentamycin (512 μg/mL), and erythromycin (256 μg/mL), indicating that it is more potent than commonly administered antibiotics [57]