A wild mushroom found on banana pseudo-stem sheath was isolated, and was identified as Volvariella
volvacea based on both morphological and molecular characteristics. The mycelia of wild strain grew on
banana leaves better than on other raw materials. Furthermore the wild strain could produce fruit bodies
on banana leaves with a biological efficiency of 26.4%, on rice straw with 23.76% and on pseudo-stem
sheath with 17.11% by a method that involves using non-pasteurized materials. This result indicates that
it is very important for the utilization of banana wastes to produce mushroom by cultivating the wild
strain.