Greater oxygen availability increased blastospore yieldwhen isolates of B. bassiana were grown under higher DOlevels (350 rpm and 50-mL volume). The main effects representedby agitation speed (ESALQ1432: F1, 24=185.65,P<0.0001; GHA: F1, 24=75.68, P<0.0001) and culture volume(ESALQ1432: F1, 24=39.19, P<0.0001; GHA: F1, 24=32.88, P<0.0001) supported a significant increase in the blastosporeconcentrations for both isolates (Table 1). Blastosporeproduction increased over time for the fermentation of ESALQ1432 (F1, 24=5.08, P=0.034), although no significantchange between days 2 and 3 was observed in GHA cultures(F1, 24=0.97, P=0.334). No significant interaction of agitationspeed and culture volume was observed for blastospore yieldsin cultures of ESALQ1432 (P>0.05), while this interactionplayed a significant role in blastospore production by culturesof GHA (F1, 24=33.46, P<0.0001)
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