Suitable mixing of nutrients and micro-organism cells greatly
affects the biochemical reaction. Diaz et al. [33] mentioned that
in static conditions as there is no physical mixing procedure therefore,
the only way of mixing is provided by the CO2 produced during
the process. The bubbles of CO2 passed through the whole medium
and thus provided a way of mixing of nutrients with cells. However,
this mixing may not be homogenous. On the other hand, in
shaking conditions, besides mixing through CO2 physical mixing is
also provided by a proper shaking of the vessel which ultimately
results in a homogenous supply of nutrients to the cells and as a
result a higher fermentation rate