if additional presumptive tube or bottle show active fermentation or acidic reaction at the end of a 48±3 h in cubation period, submit ths to the confirm phase. Simultaneous inoculation into brilliant green lactose bile broth for tatal coliform and EC broth for thermotolerant (fecal) coliform or EC-MUG broth for E.coli may be use. To confirm presumptive coliform colony growing on a solid medium using fermentation media