All fermentation inocula were prepared in stationary
cultivations incubated at 30 ◦C. Fermentations were
initiated with a 10% (v/v) inoculum. Experiments
were conducted in a 2-l controlled fermenter using
a working volume of 1 l with an agitation rate of
200 rpm, at 30 ◦C, and a pH of 5. Control of pH
was provided by the automatic addition of 3 MNaOH.
Samples were removed at various times and stored at
−20 ◦C until sample analysis.