Since long lived plants may develop
mutants even in apical meristem
(Klekowski 1985) and during the last few
years, variations have been detected among
in vitro date palm cultivars Barhi, Medjool
and Khalas such as Dwarfism, delay in
fruiting and fruiting set failure (McCubbin
et al. 2004, Al-Kaabi et al. 2005). All these
cases would greatly affect the utilization of
tissue culture techniques. Various
techniques were used to confirm the true to
typeness produced in vitro. Among the
different techniques used in generating
molecular markers, for direct detection of
genomic variation at DNA level, Random
Amplified Polymorphic DNA (RAPD)
markers have been successfully used for the
cultivar analysis and species identification
in most plants, due to the technical
simplicity and speed of the methodology
(Bader et al. 2007, Al-Khalifah and Askari
2007
Since long lived plants may developmutants even in apical meristem(Klekowski 1985) and during the last fewyears, variations have been detected amongin vitro date palm cultivars Barhi, Medjooland Khalas such as Dwarfism, delay infruiting and fruiting set failure (McCubbinet al. 2004, Al-Kaabi et al. 2005). All thesecases would greatly affect the utilization oftissue culture techniques. Varioustechniques were used to confirm the true totypeness produced in vitro. Among thedifferent techniques used in generatingmolecular markers, for direct detection ofgenomic variation at DNA level, RandomAmplified Polymorphic DNA (RAPD)markers have been successfully used for thecultivar analysis and species identificationin most plants, due to the technicalsimplicity and speed of the methodology(Bader et al. 2007, Al-Khalifah and Askari2007
การแปล กรุณารอสักครู่..
