2.1.2. The starches
(A)
Isolation and purification of bean starch: pulverized seeds were defatted by means of the Soxhlet method and then soaked in 0.005 M NaHSO3. The supernatant was decanted and the precipitate was submitted to several washes with: 0.1 N NaCl:0.1 N NaCl:toluene (140:1), 96% ethanol:water (1:3), (2:3) and (3:1) successively. Finally, it was washed with 96% ethanol, acetone and ether, then dried and weighed.